Murray, who is ranked world number two, announced in September he would donate 50 pounds for every ace he hit until the end of 2015.
He is the latest celebrity to donate money to Syrian war victims. Last month, actors Sacha Baron Cohen and Isla Fisher announced a $1 million donation to two charities working in Syria.
"I remember watching the news last summer and deciding I wanted to do something to help some of the millions of children whose lives have turned upside down by the situation in Syria," said Murray, who is expecting his first child next month with his wife Kim.
"It's amazing that we've been able to raise so much, and I'm very grateful to my sponsors and fans for their help in this – I couldn't have done it without them," he said in statement reported by Reuters.
Murray's donations to UNICEF were matched by his sponsors Standard Life and Under Armour, along with the Lawn Tennis Association and The Association of Tennis Professionals.
"Almost five years into the conflict, Syria remains one of the most dangerous places to be a child and millions of children have lost loved ones, homes and schools," UNICEF UK executive director, David Bull, said.
"Their world has been turned upside down by the conflict and humanitarian needs are becoming ever more urgent. The support of Andy, his fans and the wider tennis community will help us provide support for children who have lost so much."