Footage shows the Real Madrid star Cristiano Ronaldo coming to the window and politely asking them to keep the noise down, with Ronaldo even gesturing with a 'go to sleep' motion.
Iran and Portugal are set to square off on Monday, with Iran’s only chance of advancing to the Round of 16 coming via a win.
Portugal only needs a draw, but also aims to take the top position in Group B ahead of Spain.
In March, PSG fans attempted to disrupt Ronaldo's sleep before their Champions League clash by making noise outside the Real Madrid hotel.
