Alireza Mansourian’s side will meet Al Nassr at the Al Maktoum Stadium on Monday in Group A of the competition.
The Saudi Arabian football team will look to bounce back from its opening-day defeat to Al Wasl when it faces Zob Ahan.
In its first game in the competition since 2016, Al Nassr went down 1-0 in to Al Wasl, also in Dubai, and the Riyadh side has now gone four games on the continent without registering a goal.
Al Nassr has, however, been on a superb run domestically and scored deep into stoppage time to secure a 3-2 win against Ettifaq on Friday and move within four points of Saudi Pro League leader Al Hilal.
Zob Ahan dominated large spells of its opening match against Iraq’s Al Zawraa last week but was unable to break the deadlock as the sides shared a goalless draw.
The Isfahan team has since won its first Iranian Professional League game since August when it defeated Padideh 1-0 on Thursday to edge away from the relegation zone.
Al Nassr and Zob Ahan also met twice in the 2016 group stage with the Iranian side posting back-to-back 3-0 victories as they advanced to the knockout rounds and the Saudi side was eliminated.