Strict security arrangements were made as the PPP leaders arrived in the court along with their lawyers.
The application filed by Asif Ali Zardari and Faryal Talpur stated that the banking court's decision was not in accordance with law, requesting the Sindh High Court to declare the ruling null and void.
The banking court on Friday ordered to transfer the mega money laundering cases against the brother sister duo to a court in Islamabad, The News International reported.
The NAB has also summoned Asif Ali Zardari Bilawal Bhutto Zardai on March 20.
The Federal Investigation Agency (FIA) has been probing the money laundering case involving fake bank accounts and have arrested Omni Group owner Anwar Majeed and owner of a private bank during the course of investigation.
The FIA on July 6 last year filed a case in the banking court which conducted the hearing for eight months.
Asif Ali Zardari and Faryal Talpur have acquired protective bail to avoid arrest.