The Iranian football team has already missed Vouria Ghafouri, Humam Tareq and Mohsen Karimi for the match against Al Hilal.
The match is scheduled for Monday in Doha, Qatar.
Esteghlal is bottom of the group with just one point.
The two giants of Asian football have met on five occasions in the competition, with Esteghlal winning three times, and Al Hilal winning on two occasions.
Their most recent encounter last year ended with a 1-0 Iranian victory in Kuwait City.