Francis prayed for the victims of the strike, which on Wednesday killed at least 44 people and wounded dozens, in what the UN said could amount to a war crime, AP reported.
The pontiff also said he hoped "humanitarian corridors for the most needing migrants are organized in a concerted and extensive way."
He also remembered the victims of bloodsheds that recently took place in Afghanistan, Mali, Burkina Faso and Niger.
The Libyan attack raised new questions about the European Union's policy of cooperating with the militias that hold migrants in crowded detention centers to prevent them from crossing the Mediterranean.