Orlando Police Chief Eric Smith told reporters that the shooting followed a fight in the area of Wall Street Plaza and South Orange Avenue at approximately 2:00 a.m. (0600 GMT) as the bars and restaurants were closing, Xinhua reported.
An unidentified shooter pulled out a handgun and fired into the crowd, injuring seven people.
All the victims are hospitalized and in stable condition, Smith said.
Police are seeking information about the suspect.
The United States has suffered at least 382 mass shootings so far this year, according to the latest data from the non-profit Gun Violence Archive.