With Al Nassr of Saudi Arabia having already confirmed the Group E top spot, the only route open for Persepolis is by finishing among the three best second-placed teams from the West Zone.
Last week's goalless draw with Al Nassr took two-time AFC Champions League runner-up Persepolis to eight points.
The Iranian champion know a win against Al Duhail may eventually prove not enough but with their fans roaring the team on at the Azadi Stadium, Persepolis will be determined to take maximum points.
For Al Duhail, the tie is a chance to end a difficult campaign on a high note.
AFC Champions League semi-finalist for the first time last season, the Qatar side only has four points on the board and defeating Persepolis in Tehran will help soothe some of the pain it has endured in a tough 2023-24 season.