The match will be held at the Amman International Stadium in Amman, Jordan.
Tajikistan’s Istiklol will also host Emirati club Sharjah in another match in the group.
Al Wehdat has kicked off the domestic season strongly. Sepahan has also been impressive in the Iran Pro League - four wins on the trot giving the team a five-point lead at the top.
The top two teams from each group qualify for the knockout stage.
The 2024–25 AFC Champions League Two group stage will be played from September 17 to December 5, 2024.