The Iran side defeated Turkmenistan's Ahal 1-0 on Matchday Three, with Kaveh Rezaei scoring the winner in the 82nd minute.
Sepahan - with their fans backing them - will be confident of doing the double over Ahal, with victory for the Iranian side also confirming Al Hussein's progress into the Round of 16 from the three-team group.
The match will be held at the Naghsh-E-Jahan Stadium in Isfahan.