A woeful run means Nasaf is anchoring the West Zone standings while unbeaten Tractor is flying high with eight points on the board.
Nasaf's difficulties on the continental stage have been compounded by its troubles at home, with the Uzbekistan Super League title in danger of slipping from its grip, the-afc.com reported.
I has, however, won its two previous encounters with Iran's Tractor but the fact that it has been brittle both home and away in the AFC Champions League Elite this season will weigh heavily on its minds.
Tractor will be seeking a third consecutive win after having opened its campaign with two draws and will head into the tie unbeaten against teams from Uzbekistan in its three most recent meetings.
A third victory on the trot will mean its longest winning streak on the continent and it will fancy its chances after finding its best form in the last two ties.
The match will be held at the Markaziy Stadium in Qarshi on Monday.