Turning Back Time: Aging Reversed in Mice
TEHRAN (Tasnim) – A team of researchers identified a new way in which cells age, and could also reverse the process in old mice whose bodies appear younger in several ways.
The discovery has implications for understanding age-related diseases including cancers, neurodegenerative disorders and diabetes.
One way all mammalian cells produce energy is via aerobic respiration, in which large molecules are broken down into smaller ones, releasing energy in the process. This mainly occurs in the mitochondria – the "powerhouses" of cells. Mitochondria carry their own genomes, but some of the cellular components needed for respiration are produced partly by the nucleus, so the two must coordinate their activities.
As we age, mitochondrial function declines, which can lead to conditions such as Alzheimer's disease and diabetes. To investigate why this decline occurs, Ana Gomes at Harvard Medical School and her colleagues compared the levels of messenger RNA (mRNA) – molecules that convey genetic information around a cell – for the cellular components needed for respiration in the skeletal muscle of 6 and 22-month-old mice.
They found that the level of the mRNA in the nucleus did not change much between the young and old mice, whereas those from the mitochondria appeared to decline with age.
Similar changes were seen in mice that lacked a protein called SIRT1 – high levels of which are associated with calorie restriction and an increased lifespan. These mice also had higher levels of a protein produced by the nucleus called hypoxia inducible factor (HIF-1α).
The team wondered if this aspect of ageing could be reversed by increasing the amount of SIRT1 in the cells. To find out if that was possible, they injected 22-month-old mice twice daily for a week with nicotinamide mono nucleotide (NMN) – a molecule known to increase levels of NAD.
At the end of the week, markers of muscular atrophy and inflammation had dropped and the mice had even developed a different muscle type more common in younger mice. Together, these features were characteristic of 6-month-old mice.
"We found that modulating this pathway can improve mitochondrial function and age-associated pathologies in old mice, and therefore it gives a new pathway to target that can reverse some aspects of ageing,” Gomes said.
"This paper clearly demonstrates that NAD+ production is a sort of 'Achilles' heel', (a lack of which) significantly contributes to ageing, and also that this problem can be ameliorated by boosting NAD+ production with key intermediates, such as NMN," Shin-Ichiro Imai, from Washington University School of Medicine in St Louis said.