The Iran side will head into its final League Stage tie on six points and out of the qualification spots, with its fate also no longer in its hands.
The 4-1 defeat to Al Hilal SFC was a body blow for two-time AFC Champions League runner-up Persepolis, with the Saudi Arabian side simply too strong on the night.
The Iranian giant, however, can't afford to have another off day as Al Nassr has also been firing on all cylinders and will be tough opponent to overcome.
Ronaldo led with two goals in the 4-0 win over UAE's Al Wasl FC as Al Nassr consolidated its third-place position in the standings.
It has an outside chance of finishing higher and will be looking for a win to end the League Stage campaign with a bang.
The match will be held at the Azadi Stadium in Tehran.