Spinning Spider Silk Now Possible

Spinning Spider Silk Now Possible

TEHRAN (Tasnim) – A team of researchers from the Swedish University of Agricultural Sciences and Karolinska Institutet has developed a method to produce artificial spider silk.

The previous attempts to produce artificial spider silk involved harsh chemicals and have resulted in fibers of limited use. But today, scientists report that they can produce kilometer long threads that for the first time resemble real spider silk.

The results were published in the journal Nature Chemical Biology.

Spider silk is an attractive material -it is well tolerated when implanted in tissues, it is light-weight but stronger than steel, and it is also biodegradable. However, spiders are difficult to keep in captivity and they spin small amounts of silk. Therefore, any large scale production must involve the use of artificial silk proteins and spinning processes. A biomimetic spinning process (that mimics nature) is probably the best way to manufacture fibers that resemble real spider silk. Until now, this has not been possible because of difficulties to obtain water soluble spider silk proteins from bacteria and other production systems, and therefore strong solvents has been used in previously described spinning processes.

Researcher Anna Rising and her colleagues Jan Johansson and Marlene Andersson at the Swedish University of Agricultural Sciences and at Karolinska Institutet have previously shown that there is an impressive pH gradient in the spider silk gland, and that this well-regulated pH gradient affects specific parts of the spider silk proteins and ensures that the fiber forms rapidly in a defined place of the silk production apparatus.

This knowledge has now been used to design an artificial spider silk protein that can be produced in large quantities in bacteria, which makes the production scalable and interesting from an industrial perspective.

"To our surprise, this artificial protein is as water soluble as the natural spider silk proteins, which means that it is possible to keep the proteins soluble at extreme concentrations," Anna Rising said.

To mimic the spider silk gland, the research team constructed a simple but very efficient and biomimetic spinning apparatus in which they can spin kilometer-long fibers only by lowering the pH.

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